منابع مشابه
Microchip-based homogeneous immunoassay using a cloned enzyme donor.
We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme beta-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 microg mL(-1). Our CEDIA using a mic...
متن کاملMicrochip-based trapped-atom clocks
A two-level quantum system in vacuum—in the absence of any perturbing fields—constitutes an ideal clock whose oscillation frequency ω = E/h̄ is given by the energy difference E between the two levels |1〉 , |2〉. In a single measurement of duration T performed on a single particle this frequency can be determined with an uncertainty ∆ω = 1/T. If the measurement is performed simultaneously on N ind...
متن کاملPower-free microchip immunoassay of PSA in human serum for point-of-care testing.
For point-of-care testing (POCT), a sandwich-typed immunoassay on poly(dimethylsiloxane) (PDMS) microfluidic chips was developed by employing two original technologies: a power-free microchip and laminar flow-assisted dendritic amplification (LFDA). Sequential injection of reagents of immunoassay was carried out by the power-free microchip. In addition, for the amplification of a fluorescent si...
متن کاملDevelopment of a microchip Europium nanoparticle immunoassay for sensitive point-of-care HIV detection.
Rapid, sensitive and specific diagnostic assays play an indispensable role in determination of HIV infection stages and evaluation of efficacy of antiretroviral therapy. Recently, our laboratory developed a sensitive Europium nanoparticle-based microtiter-plate immunoassay capable of detecting target analytes at subpicogram per milliliter levels without the use of catalytic enzymes and signal a...
متن کاملOne-way multiplexed immunoassay strategy for simultaneous determination of multi-analytes by microchip electrophoresis.
An integrated microchip electrophoresis (MCE) system with online immunoreaction and laser induced fluorescence (LIF) detection has been developed for simultaneous determination of multi-analytes. In this system, the multiplexed immunoreactions between multiple antibody-immobilized glass beads with analytes and respective fluorescently labeled antigens were performed in a sample reservoir. After...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 2007
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.56.521